instat version 7.00 for windows 10 Search Results


femtojet microinjection system  (Eppendorf AG)
99
Eppendorf AG femtojet microinjection system
Femtojet Microinjection System, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/instat+version+7%2E00+for+windows+10/Eppendorf/pmc12825922-235-27-30
Average 99 stars, based on 1 article reviews
femtojet microinjection system - by Bioz Stars, 2026-09
99/100 stars
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li-7500 open path co2/h2o gas analyzer  (LI-COR Environmental)
99
LI-COR Environmental li-7500 open path co2/h2o gas analyzer
Li 7500 Open Path Co2/H2o Gas Analyzer, supplied by LI-COR Environmental, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/instat+version+7%2E00+for+windows+10/LI-7500+Open+Path+CO2%2FH2O+Gas+Analyzer/custom%40li-7500-open-path-co2-h2o-gas-analyzer%4010%2E1016%2Fj%2Eagrformet%2E2007%2E11%2E006
Average 99 stars, based on 1 article reviews
li-7500 open path co2/h2o gas analyzer - by Bioz Stars, 2026-09
99/100 stars
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instant ocean  (Aquarium Systems Inc)
90
Aquarium Systems Inc instant ocean
Instant Ocean, supplied by Aquarium Systems Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/instat+version+7%2E00+for+windows+10/instant+ocean/pmc03034104-250-75-77
Average 90 stars, based on 1 article reviews
instant ocean - by Bioz Stars, 2026-09
90/100 stars
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digital micrograph software v 1 93 1362  (Gatan Inc)
97
Gatan Inc digital micrograph software v 1 93 1362
BLM deficiency is associated with mitochondrial fragmentation and persistent activation of mitochondrial fission protein Drp1. ( A ) Confocal microscopy images showing mitochondrial morphology in fixed GM00637 ( BLM +/+ ) and BLM-deficient KSVS1452 ( BLM KO ) cells stained with Mitotracker (red) and DAPI (blue). Scale bars 10 µm. ( B ) Measurements of mitochondrial network morphology parameters using the Mito-Morphology Macro in ImageJ (version 1.53a; freely available at http://imageJ.nih.gov/ij ). ( C ) Mitochondria of cells prepared by high-pressure freezing/freeze substitution fixation (HPF/FS) were imaged by transmission electron microscopy. Sections of two representative cells of each cell line are shown at higher (scale bar 200 nm) and lower (scale bar 0.5 µm) magnification. Images were obtained with Digital Micrograph software v. 1.93.1362 (Gatan Microscopy Suite (GMS) software, https://www.gatan.com/installation-instructions#Step1 ) ( D ) Western blot analysis and quantification of expression levels from whole cell extracts of fission marker Drp1 ([6Z-82], SCBT) and fusion markers Mfn1 ([D-10], SCBT) and Opa1 ([612606], BD Biosciences) in BLM-proficient cell lines GM00637 ( BLM +/+ ) and KSVS1454 ( BLM KO/ + ) and in BLM-deficient KSVS1452 ( BLM KO ) cells. The three cell lines are isogenic; KSVS1454 ( BLM KO/ + ) was generated from KSVS1452 ( BLM KO ) by stable transfection with BLM cDNA (Fig. S1G). Whole cell extracts were prepared from three independent cultures and quantification was performed using ImageJ (version 1.53a; freely available at http://imageJ.nih.gov/ij ). Ran ([610340], BD Biosciences) was used as a loading control. ( E ) Western blot analysis of Drp1 phosphorylation status in the BLM-proficient GM00637 ( BLM + / + ) and BLM-deficient KSVS1452 ( BLM KO ) cell lines following G2/M arrest and release. Cells were blocked at G2/M with nocodazole, released for 8 h and expression levels and phosphorylation status of Drp1 analyzed by Western blot using Drp1 antibody 6Z-82 (SCBT) and phospho-Drp1-Ser616 antibody D9A1 (Cell Signaling). Ran ([610340], BD Biosciences) was used as a loading control. Significance of differences was determined by a Student’s t test and is reported as * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001.
Digital Micrograph Software V 1 93 1362, supplied by Gatan Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/instat+version+7%2E00+for+windows+10/DigitalMicrograph+Software/pmc07835382-117-4-9
Average 97 stars, based on 1 article reviews
digital micrograph software v 1 93 1362 - by Bioz Stars, 2026-09
97/100 stars
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li cor odyssey clx imaging system  (LI-COR)
99
LI-COR li cor odyssey clx imaging system
BLM deficiency is associated with mitochondrial fragmentation and persistent activation of mitochondrial fission protein Drp1. ( A ) Confocal microscopy images showing mitochondrial morphology in fixed GM00637 ( BLM +/+ ) and BLM-deficient KSVS1452 ( BLM KO ) cells stained with Mitotracker (red) and DAPI (blue). Scale bars 10 µm. ( B ) Measurements of mitochondrial network morphology parameters using the Mito-Morphology Macro in ImageJ (version 1.53a; freely available at http://imageJ.nih.gov/ij ). ( C ) Mitochondria of cells prepared by high-pressure freezing/freeze substitution fixation (HPF/FS) were imaged by transmission electron microscopy. Sections of two representative cells of each cell line are shown at higher (scale bar 200 nm) and lower (scale bar 0.5 µm) magnification. Images were obtained with Digital Micrograph software v. 1.93.1362 (Gatan Microscopy Suite (GMS) software, https://www.gatan.com/installation-instructions#Step1 ) ( D ) Western blot analysis and quantification of expression levels from whole cell extracts of fission marker Drp1 ([6Z-82], SCBT) and fusion markers Mfn1 ([D-10], SCBT) and Opa1 ([612606], BD Biosciences) in BLM-proficient cell lines GM00637 ( BLM +/+ ) and KSVS1454 ( BLM KO/ + ) and in BLM-deficient KSVS1452 ( BLM KO ) cells. The three cell lines are isogenic; KSVS1454 ( BLM KO/ + ) was generated from KSVS1452 ( BLM KO ) by stable transfection with BLM cDNA (Fig. S1G). Whole cell extracts were prepared from three independent cultures and quantification was performed using ImageJ (version 1.53a; freely available at http://imageJ.nih.gov/ij ). Ran ([610340], BD Biosciences) was used as a loading control. ( E ) Western blot analysis of Drp1 phosphorylation status in the BLM-proficient GM00637 ( BLM + / + ) and BLM-deficient KSVS1452 ( BLM KO ) cell lines following G2/M arrest and release. Cells were blocked at G2/M with nocodazole, released for 8 h and expression levels and phosphorylation status of Drp1 analyzed by Western blot using Drp1 antibody 6Z-82 (SCBT) and phospho-Drp1-Ser616 antibody D9A1 (Cell Signaling). Ran ([610340], BD Biosciences) was used as a loading control. Significance of differences was determined by a Student’s t test and is reported as * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001.
Li Cor Odyssey Clx Imaging System, supplied by LI-COR, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/instat+version+7%2E00+for+windows+10/Odyssey+CLx+Imaging+System/pm32997975-231-33-33
Average 99 stars, based on 1 article reviews
li cor odyssey clx imaging system - by Bioz Stars, 2026-09
99/100 stars
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j 1500 spectrometer  (JASCO Inc)
96
JASCO Inc j 1500 spectrometer
BLM deficiency is associated with mitochondrial fragmentation and persistent activation of mitochondrial fission protein Drp1. ( A ) Confocal microscopy images showing mitochondrial morphology in fixed GM00637 ( BLM +/+ ) and BLM-deficient KSVS1452 ( BLM KO ) cells stained with Mitotracker (red) and DAPI (blue). Scale bars 10 µm. ( B ) Measurements of mitochondrial network morphology parameters using the Mito-Morphology Macro in ImageJ (version 1.53a; freely available at http://imageJ.nih.gov/ij ). ( C ) Mitochondria of cells prepared by high-pressure freezing/freeze substitution fixation (HPF/FS) were imaged by transmission electron microscopy. Sections of two representative cells of each cell line are shown at higher (scale bar 200 nm) and lower (scale bar 0.5 µm) magnification. Images were obtained with Digital Micrograph software v. 1.93.1362 (Gatan Microscopy Suite (GMS) software, https://www.gatan.com/installation-instructions#Step1 ) ( D ) Western blot analysis and quantification of expression levels from whole cell extracts of fission marker Drp1 ([6Z-82], SCBT) and fusion markers Mfn1 ([D-10], SCBT) and Opa1 ([612606], BD Biosciences) in BLM-proficient cell lines GM00637 ( BLM +/+ ) and KSVS1454 ( BLM KO/ + ) and in BLM-deficient KSVS1452 ( BLM KO ) cells. The three cell lines are isogenic; KSVS1454 ( BLM KO/ + ) was generated from KSVS1452 ( BLM KO ) by stable transfection with BLM cDNA (Fig. S1G). Whole cell extracts were prepared from three independent cultures and quantification was performed using ImageJ (version 1.53a; freely available at http://imageJ.nih.gov/ij ). Ran ([610340], BD Biosciences) was used as a loading control. ( E ) Western blot analysis of Drp1 phosphorylation status in the BLM-proficient GM00637 ( BLM + / + ) and BLM-deficient KSVS1452 ( BLM KO ) cell lines following G2/M arrest and release. Cells were blocked at G2/M with nocodazole, released for 8 h and expression levels and phosphorylation status of Drp1 analyzed by Western blot using Drp1 antibody 6Z-82 (SCBT) and phospho-Drp1-Ser616 antibody D9A1 (Cell Signaling). Ran ([610340], BD Biosciences) was used as a loading control. Significance of differences was determined by a Student’s t test and is reported as * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001.
J 1500 Spectrometer, supplied by JASCO Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/instat+version+7%2E00+for+windows+10/J-1500/10__3762_slash_bjoc__15__164-130-8-7
Average 96 stars, based on 1 article reviews
j 1500 spectrometer - by Bioz Stars, 2026-09
96/100 stars
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polycaprolactone pcl  (Perstorp Chemicals GmbH)
90
Perstorp Chemicals GmbH polycaprolactone pcl
BLM deficiency is associated with mitochondrial fragmentation and persistent activation of mitochondrial fission protein Drp1. ( A ) Confocal microscopy images showing mitochondrial morphology in fixed GM00637 ( BLM +/+ ) and BLM-deficient KSVS1452 ( BLM KO ) cells stained with Mitotracker (red) and DAPI (blue). Scale bars 10 µm. ( B ) Measurements of mitochondrial network morphology parameters using the Mito-Morphology Macro in ImageJ (version 1.53a; freely available at http://imageJ.nih.gov/ij ). ( C ) Mitochondria of cells prepared by high-pressure freezing/freeze substitution fixation (HPF/FS) were imaged by transmission electron microscopy. Sections of two representative cells of each cell line are shown at higher (scale bar 200 nm) and lower (scale bar 0.5 µm) magnification. Images were obtained with Digital Micrograph software v. 1.93.1362 (Gatan Microscopy Suite (GMS) software, https://www.gatan.com/installation-instructions#Step1 ) ( D ) Western blot analysis and quantification of expression levels from whole cell extracts of fission marker Drp1 ([6Z-82], SCBT) and fusion markers Mfn1 ([D-10], SCBT) and Opa1 ([612606], BD Biosciences) in BLM-proficient cell lines GM00637 ( BLM +/+ ) and KSVS1454 ( BLM KO/ + ) and in BLM-deficient KSVS1452 ( BLM KO ) cells. The three cell lines are isogenic; KSVS1454 ( BLM KO/ + ) was generated from KSVS1452 ( BLM KO ) by stable transfection with BLM cDNA (Fig. S1G). Whole cell extracts were prepared from three independent cultures and quantification was performed using ImageJ (version 1.53a; freely available at http://imageJ.nih.gov/ij ). Ran ([610340], BD Biosciences) was used as a loading control. ( E ) Western blot analysis of Drp1 phosphorylation status in the BLM-proficient GM00637 ( BLM + / + ) and BLM-deficient KSVS1452 ( BLM KO ) cell lines following G2/M arrest and release. Cells were blocked at G2/M with nocodazole, released for 8 h and expression levels and phosphorylation status of Drp1 analyzed by Western blot using Drp1 antibody 6Z-82 (SCBT) and phospho-Drp1-Ser616 antibody D9A1 (Cell Signaling). Ran ([610340], BD Biosciences) was used as a loading control. Significance of differences was determined by a Student’s t test and is reported as * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001.
Polycaprolactone Pcl, supplied by Perstorp Chemicals GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/instat+version+7%2E00+for+windows+10/pcl+capa+6800/pm37242886-58-125-127
Average 90 stars, based on 1 article reviews
polycaprolactone pcl - by Bioz Stars, 2026-09
90/100 stars
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sony sx90 camera  (Sony)
90
Sony sony sx90 camera
BLM deficiency is associated with mitochondrial fragmentation and persistent activation of mitochondrial fission protein Drp1. ( A ) Confocal microscopy images showing mitochondrial morphology in fixed GM00637 ( BLM +/+ ) and BLM-deficient KSVS1452 ( BLM KO ) cells stained with Mitotracker (red) and DAPI (blue). Scale bars 10 µm. ( B ) Measurements of mitochondrial network morphology parameters using the Mito-Morphology Macro in ImageJ (version 1.53a; freely available at http://imageJ.nih.gov/ij ). ( C ) Mitochondria of cells prepared by high-pressure freezing/freeze substitution fixation (HPF/FS) were imaged by transmission electron microscopy. Sections of two representative cells of each cell line are shown at higher (scale bar 200 nm) and lower (scale bar 0.5 µm) magnification. Images were obtained with Digital Micrograph software v. 1.93.1362 (Gatan Microscopy Suite (GMS) software, https://www.gatan.com/installation-instructions#Step1 ) ( D ) Western blot analysis and quantification of expression levels from whole cell extracts of fission marker Drp1 ([6Z-82], SCBT) and fusion markers Mfn1 ([D-10], SCBT) and Opa1 ([612606], BD Biosciences) in BLM-proficient cell lines GM00637 ( BLM +/+ ) and KSVS1454 ( BLM KO/ + ) and in BLM-deficient KSVS1452 ( BLM KO ) cells. The three cell lines are isogenic; KSVS1454 ( BLM KO/ + ) was generated from KSVS1452 ( BLM KO ) by stable transfection with BLM cDNA (Fig. S1G). Whole cell extracts were prepared from three independent cultures and quantification was performed using ImageJ (version 1.53a; freely available at http://imageJ.nih.gov/ij ). Ran ([610340], BD Biosciences) was used as a loading control. ( E ) Western blot analysis of Drp1 phosphorylation status in the BLM-proficient GM00637 ( BLM + / + ) and BLM-deficient KSVS1452 ( BLM KO ) cell lines following G2/M arrest and release. Cells were blocked at G2/M with nocodazole, released for 8 h and expression levels and phosphorylation status of Drp1 analyzed by Western blot using Drp1 antibody 6Z-82 (SCBT) and phospho-Drp1-Ser616 antibody D9A1 (Cell Signaling). Ran ([610340], BD Biosciences) was used as a loading control. Significance of differences was determined by a Student’s t test and is reported as * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001.
Sony Sx90 Camera, supplied by Sony, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/instat+version+7%2E00+for+windows+10/digital+camera+sony+dsc+s90/us11135267-483-11-10
Average 90 stars, based on 1 article reviews
sony sx90 camera - by Bioz Stars, 2026-09
90/100 stars
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rabbit anti cleaved caspase 3  (Cell Signaling Technology Inc)
99
Cell Signaling Technology Inc rabbit anti cleaved caspase 3
BLM deficiency is associated with mitochondrial fragmentation and persistent activation of mitochondrial fission protein Drp1. ( A ) Confocal microscopy images showing mitochondrial morphology in fixed GM00637 ( BLM +/+ ) and BLM-deficient KSVS1452 ( BLM KO ) cells stained with Mitotracker (red) and DAPI (blue). Scale bars 10 µm. ( B ) Measurements of mitochondrial network morphology parameters using the Mito-Morphology Macro in ImageJ (version 1.53a; freely available at http://imageJ.nih.gov/ij ). ( C ) Mitochondria of cells prepared by high-pressure freezing/freeze substitution fixation (HPF/FS) were imaged by transmission electron microscopy. Sections of two representative cells of each cell line are shown at higher (scale bar 200 nm) and lower (scale bar 0.5 µm) magnification. Images were obtained with Digital Micrograph software v. 1.93.1362 (Gatan Microscopy Suite (GMS) software, https://www.gatan.com/installation-instructions#Step1 ) ( D ) Western blot analysis and quantification of expression levels from whole cell extracts of fission marker Drp1 ([6Z-82], SCBT) and fusion markers Mfn1 ([D-10], SCBT) and Opa1 ([612606], BD Biosciences) in BLM-proficient cell lines GM00637 ( BLM +/+ ) and KSVS1454 ( BLM KO/ + ) and in BLM-deficient KSVS1452 ( BLM KO ) cells. The three cell lines are isogenic; KSVS1454 ( BLM KO/ + ) was generated from KSVS1452 ( BLM KO ) by stable transfection with BLM cDNA (Fig. S1G). Whole cell extracts were prepared from three independent cultures and quantification was performed using ImageJ (version 1.53a; freely available at http://imageJ.nih.gov/ij ). Ran ([610340], BD Biosciences) was used as a loading control. ( E ) Western blot analysis of Drp1 phosphorylation status in the BLM-proficient GM00637 ( BLM + / + ) and BLM-deficient KSVS1452 ( BLM KO ) cell lines following G2/M arrest and release. Cells were blocked at G2/M with nocodazole, released for 8 h and expression levels and phosphorylation status of Drp1 analyzed by Western blot using Drp1 antibody 6Z-82 (SCBT) and phospho-Drp1-Ser616 antibody D9A1 (Cell Signaling). Ran ([610340], BD Biosciences) was used as a loading control. Significance of differences was determined by a Student’s t test and is reported as * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001.
Rabbit Anti Cleaved Caspase 3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/instat+version+7%2E00+for+windows+10/Caspase-3+Antibody/pm25347746-209-35-39
Average 99 stars, based on 1 article reviews
rabbit anti cleaved caspase 3 - by Bioz Stars, 2026-09
99/100 stars
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flowcam instrument: model vs1, serial #551  (Sony)
90
Sony flowcam instrument: model vs1, serial #551
BLM deficiency is associated with mitochondrial fragmentation and persistent activation of mitochondrial fission protein Drp1. ( A ) Confocal microscopy images showing mitochondrial morphology in fixed GM00637 ( BLM +/+ ) and BLM-deficient KSVS1452 ( BLM KO ) cells stained with Mitotracker (red) and DAPI (blue). Scale bars 10 µm. ( B ) Measurements of mitochondrial network morphology parameters using the Mito-Morphology Macro in ImageJ (version 1.53a; freely available at http://imageJ.nih.gov/ij ). ( C ) Mitochondria of cells prepared by high-pressure freezing/freeze substitution fixation (HPF/FS) were imaged by transmission electron microscopy. Sections of two representative cells of each cell line are shown at higher (scale bar 200 nm) and lower (scale bar 0.5 µm) magnification. Images were obtained with Digital Micrograph software v. 1.93.1362 (Gatan Microscopy Suite (GMS) software, https://www.gatan.com/installation-instructions#Step1 ) ( D ) Western blot analysis and quantification of expression levels from whole cell extracts of fission marker Drp1 ([6Z-82], SCBT) and fusion markers Mfn1 ([D-10], SCBT) and Opa1 ([612606], BD Biosciences) in BLM-proficient cell lines GM00637 ( BLM +/+ ) and KSVS1454 ( BLM KO/ + ) and in BLM-deficient KSVS1452 ( BLM KO ) cells. The three cell lines are isogenic; KSVS1454 ( BLM KO/ + ) was generated from KSVS1452 ( BLM KO ) by stable transfection with BLM cDNA (Fig. S1G). Whole cell extracts were prepared from three independent cultures and quantification was performed using ImageJ (version 1.53a; freely available at http://imageJ.nih.gov/ij ). Ran ([610340], BD Biosciences) was used as a loading control. ( E ) Western blot analysis of Drp1 phosphorylation status in the BLM-proficient GM00637 ( BLM + / + ) and BLM-deficient KSVS1452 ( BLM KO ) cell lines following G2/M arrest and release. Cells were blocked at G2/M with nocodazole, released for 8 h and expression levels and phosphorylation status of Drp1 analyzed by Western blot using Drp1 antibody 6Z-82 (SCBT) and phospho-Drp1-Ser616 antibody D9A1 (Cell Signaling). Ran ([610340], BD Biosciences) was used as a loading control. Significance of differences was determined by a Student’s t test and is reported as * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001.
Flowcam Instrument: Model Vs1, Serial #551, supplied by Sony, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/instat+version+7%2E00+for+windows+10/flowcam+instrument++model+vs1++serial++551/us11135267-483-0-10
Average 90 stars, based on 1 article reviews
flowcam instrument: model vs1, serial #551 - by Bioz Stars, 2026-09
90/100 stars
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growing substrate for pre-culturing  (Chemie GmbH)
90
Chemie GmbH growing substrate for pre-culturing
BLM deficiency is associated with mitochondrial fragmentation and persistent activation of mitochondrial fission protein Drp1. ( A ) Confocal microscopy images showing mitochondrial morphology in fixed GM00637 ( BLM +/+ ) and BLM-deficient KSVS1452 ( BLM KO ) cells stained with Mitotracker (red) and DAPI (blue). Scale bars 10 µm. ( B ) Measurements of mitochondrial network morphology parameters using the Mito-Morphology Macro in ImageJ (version 1.53a; freely available at http://imageJ.nih.gov/ij ). ( C ) Mitochondria of cells prepared by high-pressure freezing/freeze substitution fixation (HPF/FS) were imaged by transmission electron microscopy. Sections of two representative cells of each cell line are shown at higher (scale bar 200 nm) and lower (scale bar 0.5 µm) magnification. Images were obtained with Digital Micrograph software v. 1.93.1362 (Gatan Microscopy Suite (GMS) software, https://www.gatan.com/installation-instructions#Step1 ) ( D ) Western blot analysis and quantification of expression levels from whole cell extracts of fission marker Drp1 ([6Z-82], SCBT) and fusion markers Mfn1 ([D-10], SCBT) and Opa1 ([612606], BD Biosciences) in BLM-proficient cell lines GM00637 ( BLM +/+ ) and KSVS1454 ( BLM KO/ + ) and in BLM-deficient KSVS1452 ( BLM KO ) cells. The three cell lines are isogenic; KSVS1454 ( BLM KO/ + ) was generated from KSVS1452 ( BLM KO ) by stable transfection with BLM cDNA (Fig. S1G). Whole cell extracts were prepared from three independent cultures and quantification was performed using ImageJ (version 1.53a; freely available at http://imageJ.nih.gov/ij ). Ran ([610340], BD Biosciences) was used as a loading control. ( E ) Western blot analysis of Drp1 phosphorylation status in the BLM-proficient GM00637 ( BLM + / + ) and BLM-deficient KSVS1452 ( BLM KO ) cell lines following G2/M arrest and release. Cells were blocked at G2/M with nocodazole, released for 8 h and expression levels and phosphorylation status of Drp1 analyzed by Western blot using Drp1 antibody 6Z-82 (SCBT) and phospho-Drp1-Ser616 antibody D9A1 (Cell Signaling). Ran ([610340], BD Biosciences) was used as a loading control. Significance of differences was determined by a Student’s t test and is reported as * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001.
Growing Substrate For Pre Culturing, supplied by Chemie GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/instat+version+7%2E00+for+windows+10/growing+substrate+for+pre+culturing/pmc11020074__DataSheet_6-7-215-173
Average 90 stars, based on 1 article reviews
growing substrate for pre-culturing - by Bioz Stars, 2026-09
90/100 stars
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neuralnet package  (myDATA GmbH)
90
myDATA GmbH neuralnet package
BLM deficiency is associated with mitochondrial fragmentation and persistent activation of mitochondrial fission protein Drp1. ( A ) Confocal microscopy images showing mitochondrial morphology in fixed GM00637 ( BLM +/+ ) and BLM-deficient KSVS1452 ( BLM KO ) cells stained with Mitotracker (red) and DAPI (blue). Scale bars 10 µm. ( B ) Measurements of mitochondrial network morphology parameters using the Mito-Morphology Macro in ImageJ (version 1.53a; freely available at http://imageJ.nih.gov/ij ). ( C ) Mitochondria of cells prepared by high-pressure freezing/freeze substitution fixation (HPF/FS) were imaged by transmission electron microscopy. Sections of two representative cells of each cell line are shown at higher (scale bar 200 nm) and lower (scale bar 0.5 µm) magnification. Images were obtained with Digital Micrograph software v. 1.93.1362 (Gatan Microscopy Suite (GMS) software, https://www.gatan.com/installation-instructions#Step1 ) ( D ) Western blot analysis and quantification of expression levels from whole cell extracts of fission marker Drp1 ([6Z-82], SCBT) and fusion markers Mfn1 ([D-10], SCBT) and Opa1 ([612606], BD Biosciences) in BLM-proficient cell lines GM00637 ( BLM +/+ ) and KSVS1454 ( BLM KO/ + ) and in BLM-deficient KSVS1452 ( BLM KO ) cells. The three cell lines are isogenic; KSVS1454 ( BLM KO/ + ) was generated from KSVS1452 ( BLM KO ) by stable transfection with BLM cDNA (Fig. S1G). Whole cell extracts were prepared from three independent cultures and quantification was performed using ImageJ (version 1.53a; freely available at http://imageJ.nih.gov/ij ). Ran ([610340], BD Biosciences) was used as a loading control. ( E ) Western blot analysis of Drp1 phosphorylation status in the BLM-proficient GM00637 ( BLM + / + ) and BLM-deficient KSVS1452 ( BLM KO ) cell lines following G2/M arrest and release. Cells were blocked at G2/M with nocodazole, released for 8 h and expression levels and phosphorylation status of Drp1 analyzed by Western blot using Drp1 antibody 6Z-82 (SCBT) and phospho-Drp1-Ser616 antibody D9A1 (Cell Signaling). Ran ([610340], BD Biosciences) was used as a loading control. Significance of differences was determined by a Student’s t test and is reported as * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001.
Neuralnet Package, supplied by myDATA GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/instat+version+7%2E00+for+windows+10/neuralnet+package/pmc08375798-51-172-169
Average 90 stars, based on 1 article reviews
neuralnet package - by Bioz Stars, 2026-09
90/100 stars
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BLM deficiency is associated with mitochondrial fragmentation and persistent activation of mitochondrial fission protein Drp1. ( A ) Confocal microscopy images showing mitochondrial morphology in fixed GM00637 ( BLM +/+ ) and BLM-deficient KSVS1452 ( BLM KO ) cells stained with Mitotracker (red) and DAPI (blue). Scale bars 10 µm. ( B ) Measurements of mitochondrial network morphology parameters using the Mito-Morphology Macro in ImageJ (version 1.53a; freely available at http://imageJ.nih.gov/ij ). ( C ) Mitochondria of cells prepared by high-pressure freezing/freeze substitution fixation (HPF/FS) were imaged by transmission electron microscopy. Sections of two representative cells of each cell line are shown at higher (scale bar 200 nm) and lower (scale bar 0.5 µm) magnification. Images were obtained with Digital Micrograph software v. 1.93.1362 (Gatan Microscopy Suite (GMS) software, https://www.gatan.com/installation-instructions#Step1 ) ( D ) Western blot analysis and quantification of expression levels from whole cell extracts of fission marker Drp1 ([6Z-82], SCBT) and fusion markers Mfn1 ([D-10], SCBT) and Opa1 ([612606], BD Biosciences) in BLM-proficient cell lines GM00637 ( BLM +/+ ) and KSVS1454 ( BLM KO/ + ) and in BLM-deficient KSVS1452 ( BLM KO ) cells. The three cell lines are isogenic; KSVS1454 ( BLM KO/ + ) was generated from KSVS1452 ( BLM KO ) by stable transfection with BLM cDNA (Fig. S1G). Whole cell extracts were prepared from three independent cultures and quantification was performed using ImageJ (version 1.53a; freely available at http://imageJ.nih.gov/ij ). Ran ([610340], BD Biosciences) was used as a loading control. ( E ) Western blot analysis of Drp1 phosphorylation status in the BLM-proficient GM00637 ( BLM + / + ) and BLM-deficient KSVS1452 ( BLM KO ) cell lines following G2/M arrest and release. Cells were blocked at G2/M with nocodazole, released for 8 h and expression levels and phosphorylation status of Drp1 analyzed by Western blot using Drp1 antibody 6Z-82 (SCBT) and phospho-Drp1-Ser616 antibody D9A1 (Cell Signaling). Ran ([610340], BD Biosciences) was used as a loading control. Significance of differences was determined by a Student’s t test and is reported as * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001.

Journal: Scientific Reports

Article Title: Bloom syndrome DNA helicase deficiency is associated with oxidative stress and mitochondrial network changes

doi: 10.1038/s41598-021-81075-0

Figure Lengend Snippet: BLM deficiency is associated with mitochondrial fragmentation and persistent activation of mitochondrial fission protein Drp1. ( A ) Confocal microscopy images showing mitochondrial morphology in fixed GM00637 ( BLM +/+ ) and BLM-deficient KSVS1452 ( BLM KO ) cells stained with Mitotracker (red) and DAPI (blue). Scale bars 10 µm. ( B ) Measurements of mitochondrial network morphology parameters using the Mito-Morphology Macro in ImageJ (version 1.53a; freely available at http://imageJ.nih.gov/ij ). ( C ) Mitochondria of cells prepared by high-pressure freezing/freeze substitution fixation (HPF/FS) were imaged by transmission electron microscopy. Sections of two representative cells of each cell line are shown at higher (scale bar 200 nm) and lower (scale bar 0.5 µm) magnification. Images were obtained with Digital Micrograph software v. 1.93.1362 (Gatan Microscopy Suite (GMS) software, https://www.gatan.com/installation-instructions#Step1 ) ( D ) Western blot analysis and quantification of expression levels from whole cell extracts of fission marker Drp1 ([6Z-82], SCBT) and fusion markers Mfn1 ([D-10], SCBT) and Opa1 ([612606], BD Biosciences) in BLM-proficient cell lines GM00637 ( BLM +/+ ) and KSVS1454 ( BLM KO/ + ) and in BLM-deficient KSVS1452 ( BLM KO ) cells. The three cell lines are isogenic; KSVS1454 ( BLM KO/ + ) was generated from KSVS1452 ( BLM KO ) by stable transfection with BLM cDNA (Fig. S1G). Whole cell extracts were prepared from three independent cultures and quantification was performed using ImageJ (version 1.53a; freely available at http://imageJ.nih.gov/ij ). Ran ([610340], BD Biosciences) was used as a loading control. ( E ) Western blot analysis of Drp1 phosphorylation status in the BLM-proficient GM00637 ( BLM + / + ) and BLM-deficient KSVS1452 ( BLM KO ) cell lines following G2/M arrest and release. Cells were blocked at G2/M with nocodazole, released for 8 h and expression levels and phosphorylation status of Drp1 analyzed by Western blot using Drp1 antibody 6Z-82 (SCBT) and phospho-Drp1-Ser616 antibody D9A1 (Cell Signaling). Ran ([610340], BD Biosciences) was used as a loading control. Significance of differences was determined by a Student’s t test and is reported as * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001.

Article Snippet: Images were obtained with Digital Micrograph software v. 1.93.1362 (Gatan Microscopy Suite (GMS) software, https://www.gatan.com/installation-instructions#Step1 ) ( D ) Western blot analysis and quantification of expression levels from whole cell extracts of fission marker Drp1 ([6Z-82], SCBT) and fusion markers Mfn1 ([D-10], SCBT) and Opa1 ([612606], BD Biosciences) in BLM-proficient cell lines GM00637 ( BLM +/+ ) and KSVS1454 ( BLM KO/ + ) and in BLM-deficient KSVS1452 ( BLM KO ) cells.

Techniques: Activation Assay, Confocal Microscopy, Staining, Transmission Assay, Electron Microscopy, Software, Microscopy, Western Blot, Expressing, Marker, Generated, Stable Transfection